Totipotent cells have the highest developmental potential and can only be created by nuclear transfer into oocytes. Identities of maternal factors that can induce this reprogramming remain a mystery. In this report, we demonstrate induction of totipotency on mouse embryonic stem cells by introducing six factors, Hist1h2aa, H3f3b, H1foo, p-Npm2,...
Metabolic engineering of microorganisms to produce useful compounds from renewable substrates is a promising means for sustainable, on-demand production of chemicals. However, efforts to design and engineer microbial cell factories are constrained by costly and slow “build” times in which each genetic variation requires re-engineering a new strain for each...
The limits of miniaturization of electronic device components and the steady need for faster computation power have motivated the discovery and cultivation of low-dimensional materials. Among these, two-dimensional (2D) materials have exhibited a wide range of superlative optoelectronic, thermal and mechanical properties. The interest in 2D materials took-off with the...
Mechanisms for sensing fluid flow are well studied in arthropods and in some aquatic mammals, but we know very little about how terrestrial mammals detect and localize airflow (Chapter 1). In this thesis, I will describe a series of studies in rodents that investigate the behavioral, mechanical, and neural basis...
Wind pollination has evolved from animal pollination at least 65 times in flowering plants, but the reverse transition is rare. Wind pollination is usually accompanied by the loss of traits that attract pollinators, and re-evolving them may be difficult. This dissertation examines this rare transition using Artocarpus (ca. 70 spp.,...
Literary critics typically oppose chronological and anachronistic historiographical schemes. In paired readings of early modern and contemporary poets, my dissertation, “The Shock of the Old: Periodization, Poetics, and Diachronic Exchange between the Renaissance and the Avant-Garde” investigates a series of poetic texts that defy this opposition. The poetic objects I...
What follows is a strictly post-genomic dissertation. Over the past five years, I have strived to leave the nucleus behind, and even the endoplasmic reticulum and golgi apparatus. The human genome was sequenced over 15 years before the submission of this document, and the world changed somewhat less than anticipated—besides...
This dissertation develops a framework to approach musical hybridity by considering style and genre interactions as an analytical layer of combinations of identities. It engages with the structural, contextual, and perceptual aspects of music by mixing perspectives from music studies with postcolonial studies, situated cognition, and genre studies. Hybridity is...
The rodent whisker system is a widely used model to study the sense of touch. Its neuroanatomy parallels that of the human, but its mechanics are vastly simplified compared to that of the human tactile system. Just like other modalities with complex sensor accessory structures, the geometry, mechanics, and material...