High-throughput methods enable rapid experimentation and/or screening of thousands of samples simultaneously. Mass-spectrometry based methods are of particular interest since they provide a label-free way to detect all species present in a given reaction mixture. To circumvent sample preparation and purification—which is typically a slow process—the Mrksich group developed a...
Label-free assays, and particularly those based on the combination of mass spectroscopy with surface chemistries, enable high-throughput experiments of a broad range of reactions. However, these methods can still require the incorporation of functional groups that allow immobilization of reactants and products to surfaces prior to analysis. In this thesis,...
What follows is a strictly post-genomic dissertation. Over the past five years, I have strived to leave the nucleus behind, and even the endoplasmic reticulum and golgi apparatus. The human genome was sequenced over 15 years before the submission of this document, and the world changed somewhat less than anticipated—besides...
Natural products provide a renewable resource of novel chemical structures with relevance to the pharmaceutical, agricultural and materials industries. Genome sequencing has revealed that these industries currently rely on only a fraction of the number of natural products that exist in nature. Based on the impact that known natural products...