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Dissecting the stability determinants of a challenging de novo protein fold and assay development to identify cell-penetrating miniproteins

Description:

This dissertation focuses on quantifying protein folding stability determinants and presenting initial experiments that can guide the development of a novel assay that identifies cell-penetrating miniproteins. First, despite over a century of scholarship on protein folding stability, applying this knowledge to design proteins computationally remains limited. Usually, protein designers generate...

Keyword:

protein design, cell-penetrating protein, protein folding stability, and synthetic biology

Subject:

Biology, Cellular biology, and Biophysics

Creator:

Kim, Tae-Eun

Owner:

Scholarly Digital Publishing

Language:

en

Date Uploaded:

08/23/2023

Date Created:

2023-01-01

Resource Type:

Dissertation

Alternate Identifier:

etdadmin_upload_1008136 and http://dissertations.umi.com/northwestern:16683
Dissecting the stability determinants of a challenging de novo protein fold and assay development to identify cell-penetrating miniproteins

Description:

This dissertation focuses on quantifying protein folding stability determinants and presenting initial experiments that can guide the development of a novel assay that identifies cell-penetrating miniproteins. First, despite over a century of scholarship on protein folding stability, applying this knowledge to design proteins computationally remains limited. Usually, protein designers generate...

Keyword:

protein design, cell-penetrating protein, synthetic biology, and protein folding stability

Subject:

Biology, Cellular biology, and Biophysics

Creator:

Kim, Tae-Eun

Owner:

Scholarly Digital Publishing

Language:

en

Date Uploaded:

08/23/2023

Date Created:

2023-01-01

Resource Type:

Dissertation

Alternate Identifier:

http://dissertations.umi.com/northwestern:16695 and etdadmin_upload_1009800

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Dissecting the stability determinants of a challenging de novo protein fold and assay development to identify cell-penetrating miniproteins

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