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Description:: This dissertation focuses on quantifying protein folding stability determinants and presenting initial experiments that can guide the development of a novel assay that identifies cell-penetrating miniproteins. First, despite over a century of scholarship on protein folding stability, applying this knowledge to design proteins computationally remains limited. Usually, protein designers generate...
Keyword:: protein design, cell-penetrating protein, protein folding stability, and synthetic biology
Subject:: Biology, Cellular biology, and Biophysics
Creator:: Kim, Tae-Eun
Owner:: Scholarly Digital Publishing
Language:: en
Date Uploaded:: 08/23/2023
Date Created:: 2023-01-01
Resource Type:: Dissertation
Alternate Identifier:: etdadmin_upload_1008136 and http://dissertations.umi.com/northwestern:16683

Description:: This dissertation focuses on quantifying protein folding stability determinants and presenting initial experiments that can guide the development of a novel assay that identifies cell-penetrating miniproteins. First, despite over a century of scholarship on protein folding stability, applying this knowledge to design proteins computationally remains limited. Usually, protein designers generate...
Keyword:: protein design, cell-penetrating protein, synthetic biology, and protein folding stability
Subject:: Biology, Cellular biology, and Biophysics
Creator:: Kim, Tae-Eun
Owner:: Scholarly Digital Publishing
Language:: en
Date Uploaded:: 08/23/2023
Date Created:: 2023-01-01
Resource Type:: Dissertation
Alternate Identifier:: http://dissertations.umi.com/northwestern:16695 and etdadmin_upload_1009800

Description:: Metabolic engineering of microorganisms to produce useful compounds from renewable substrates is a promising means for sustainable, on-demand production of chemicals. However, efforts to design and engineer microbial cell factories are constrained by costly and slow “build” times in which each genetic variation requires re-engineering a new strain for each...
Keyword:: metabolic engineering, cell-free, synthetic biology, prototyping, and limonene
Subject:: Chemical and Biological Engineering
Creator:: Quentin Merle Dudley
Owner:: Scholarly Digital Publishing
Date Uploaded:: 01/25/2019
Date Modified:: 01/30/2019
Date Created:: 2017-01-01
Resource Type:: Dissertation

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