Recent years have seen intense interest in the development of point-of-care nucleic acid diagnostic technologies to address the scaling limitations of laboratory-based approaches. Chief among these are combinations of isothermal amplification approaches with CRISPR-based detection and read outs of target products. Here, we contribute to the growing body of rapid, programmable point-of-care pathogen tests by developing and optimizing a one-pot NASBA-Cas13a nucleic acid detection assay. This test uses the isothermal amplification technique NASBA to amplify target viral nucleic acids, followed by Cas13a-based detection of amplified sequences. We first demonstrate an in-house formulation of NASBA that enables optimization of individual NASBA components. We then present design rules for NASBA primer sets and LbuCas13a guide RNAs for fast and sensitive detection of SARS-CoV-2 viral RNA fragments, resulting in 20–200 aM sensitivity without any specialized equipment. Finally, we explore the combination of high-throughput assay condition screening with mechanistic ordinary differential equation modeling of the reaction scheme to gain a deeper understanding of the NASBA-Cas13a system. This work presents a framework for developing a mechanistic understanding of reaction performance and optimization that uses both experiments and modeling, which we anticipate will be useful in developing future nucleic acid detection technologies.

Last modified

• 07/01/2024

Creator

• Jung, Jaeyoung K.
• Mangan, Niall M.
• Lucks, Julius B.
• Dreyer, Kathleen S.
• Shirman, Sasha
• Verosloff, Matthew S.
• Cabezas, Maria D.
• Alam, Khalid K.
• Rybnicky, Grant A.
• Bagheri, Neda
• D'Aquino, Anne E.
• Seki, Kosuke
• Jewett, Michael C.
• Muldoon, Joseph J.
• Leonard, Joshua N.
• George, Jithin
• Dray, Kate E.

DOI

• https://doi.org/10.21985/n2-bn16-9b08

Publisher

• Northwestern University

Date created

• 2024-07-01

Resource type

• Dataset

License

• Creative Commons CC0 1.0 Universal

Total Size

• 32.5 MB